Ilex Life Sciences FGF-2 (FGF-basic) Human (147 aa), E. coli Recombinant Protein (10 ug). DESCRIPTION: Our bioactive FGF-2 (FGF-basic) Human, E. coli Recombinant Protein (147 aa), also known as truncated Fibroblast Growth Factor 2, is a single, non-glycosylated, polypeptide chain with 147 amino acids and 16.5 kDa molecular mass. The FGF-2 is purified by proprietary chromatographic techniques. APPLICATION: FGF basic is a critical component of human embryonic stem cell (hESCs) culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state. FORMULATION: Sterile filtered white powder lyophilized from a sterile filtered solution containing 20mM Tris-HCl, pH 7.6 and 150mM NaCl. PURITY: Greater than 98.0% (SDS-PAGE). BIOACTIVITY: The ED50, Calculated by the dose- dependent proliferation of mouse BALB/c 3T3 cells is < 0.05 ng/ml corresponding to a specific activity of 2.0x107 units/mg. SYNONYMS: FGF-b, Basic fibroblast growth factor, bFGF, HBGF-2.